Professor, Department of
University of Maryland
Dipl. Ing (g (M.Sc.), Technische Universität Berlin,
Ph.D., Johns Hopkins University, 2000
PostDoc, Harvard Medical School 2000-2005
Instructor, Harvard Medical School 2005-2006
2007 Ralph E Powe Award
2010 Alfred P. Sloan Foundation
2013 NOHR/ARo Burt Evans Award
Dr. Kanold studies the development and plasticity of the
brain, in particular how periods of learning and plasticity are initiated and
controlled. His work focuses on the development of the central auditory and
visual system in particular on the role of early cortical circuits in brain
wiring. He uses advanced neurophysiological, in vivo imaging, optogenetic,
molecular and computational techniques. His work furthers our understanding of
how prenatal and postnatal brain injury contribute to neurodevelopmental
disorders such as cerebral palsy, epilepsy and schizophrenia.
P.O. Kanold, “Subplate neurons: crucial
regulators of cortical development and plasticity”, Frontiers in Neuroanatomy,
A. Datwani, M.J. McConnell, P.O. Kanold, K.D. Micheva, B.
Busse, M. Shamloo, S.J. Smith, and C.J. Shatz,, “Classical MHCI molecules
regulate retinogeniculate refinement and limit ocular dominance plasticity”,
Neuron, 64: 463–470, 2009
P.O. Kanold, Y.A. Kim, T. GrandPre, C. J. Shatz,
"Co-regulation of ocular dominance plasticity and NMDA receptor subunit
expression in glutamic acid decarboxylase-65 knock-out mice”, J. Physiology
(London), 587(Pt 12):2857-67. 2009
of Otolaryngology and Head & Neck Surgery
Loma Linda University
– Present, VA Loma Linda Healthcare System/ Loma Linda University
Research Scientist/ Assistant Professor
– 2015, Oregon Health and Science University
Assistant Scientist (Oregon Hearing Research Center)
– 2013, Oregon Health and Science University
Senior Research Associate
– 2008 Fred Hutchinson Cancer Research: Seattle, WA
Senior Fellow (Clinical Research Division)
– 2008 University of Washington School of Medicine: Seattle, WA
Senior Fellow (Radiation Oncology and Immunology)
2003- Sep 2004 Arizona State University: Tempe, AZ
Research Associate (Neural Coding Lab/Speech and Hearing Science)
in Neurophysiology, Arizona State University, 1997 -2003
BS in Life Science, Peking
University, 1992, 1997
Associate Professor, Cell Biology
University of Virgina
• BS, Biochemistry, Beijing University
• PhD, Biology, Massachusetts Institute of Technology
• Postdoc, Developmental Neurobiology, University of California, San Francisco
Developmental regulation of planar cell polarity in the mammalian nervous system
Cell polarity is tightly coupled to specialized cell functions and is fundamental to many cellular processes in development and disease. Two forms of epithelial polarity have been observed: one along the apical-basal axis, and one orthogonal to the apical-basal axis known as planar cell polarity (PCP). The Lu lab is interested in dissecting the signaling events that generate planar asymmetry and the resulting cellular behaviors during mammalian development, with a specific focus on two types of polarized cells in the nervous system, namely neuroepithelial cells and neurons.
It has been shown that an evolutionarily conserved noncanonical, b-catenin-independent Wnt pathway regulates PCP and is involved in diverse processes such as neural tube closure and cochlear hair cell morphogenesis. Recently, in a gene trap screen in the mouse, we identified PTK7, an atypical receptor tyrosine kinase, as a novel regulator of PCP in vertebrates. Interestingly, homologs of several vertebrate PCP genes, including PTK7, have not been implicated in PCP signaling in Drosophila, suggesting that vertebrates have evolved novel strategies to regulate PCP. Using PTK7 as an entry point, we are taking a combination of biochemical, genetic and cell biological approaches to further elucidate signaling mechanisms by which the PCP pathway exert its effect on cellular machinery, such as the cytoskeleton and protein trafficking network.
The cochlear sensory epithelium of the mouse offers an attractive system to study PCP at a single cell resolution both in vivo and in organotypic cultures. PCP is manifested by the asymmetric orientation of the hair cell stereocilia, an actin-rich structure responsible of mechanotransduction. Using a variety of genetic and molecular manipulations and imaging techniques, experiments are underway to better understand the sequence of events that lead to coordinated polarized outgrowth of stereocilia across the sensory epithelium.
We are extending our analysis of the PCP pathway into the development of the central nervous system. Our preliminary analysis of mutant phenotype suggests that PCP pathway is involved in patterning certain regions of the CNS. Currently we are investigating the molecular pathway that underlies the patterning defect. Furthermore, aided by expression studies, we hypothesize that PCP signaling also functions in later aspects of CNS development, such as cell and axon migration and target selection. We plan to use transgenic mice and in vitro neuronal cultures to test this hypothesis.
Ultimately, we hope that our studies will shed light on how the versatile PCP pathway controls cell polarity in different contexts during normal development and how mutations disrupt polarity in related human disease and birth defects
Andreeva A, Lee J, Lohia M, Wu X, Macara IG, Lu X, PTK7-Src signaling at epithelial cell contacts mediates spatial organization of actomyosin and planar cell polarity., 2014; Developmental cell. 29(1) 20-33. PMID: 24703874 | PMCID: PMC4086913
Williams M, Yen W, Lu X, Sutherland A, Distinct apical and basolateral mechanisms drive planar cell polarity-dependent convergent extension of the mouse neural plate., 2014; Developmental cell. 29(1) 34-46. PMID: 24703875 | PMCID: PMC4120093
Sipe CW, Liu L, Lee J, Grimsley-Myers C, Lu X, Lis1 mediates planar polarity of auditory hair cells through regulation of microtubule organization., 2013; Development (Cambridge, England). 140(8) 1785-95. PMID: 23533177 | PMCID: PMC3621493
Lee J, Andreeva A, Sipe CW, Liu L, Cheng A, Lu X, PTK7 regulates myosin II activity to orient planar polarity in the mammalian auditory epithelium., 2012; Current biology : CB. 22(11) 956-66. PMID: 22560610 | PMCID: PMC3407606
Sipe CW, Lu X, Kif3a regulates planar polarization of auditory hair cells through both ciliary and non-ciliary mechanisms., 2011; Development (Cambridge, England). 138(16) 3441-9. PMID: 21752934 | PMCID: PMC3143564
Paudyal A, Damrau C, Patterson VL, Ermakov A, Formstone C, Lalanne Z, Wells S, Lu X, Norris DP, Dean CH, Henderson DJ, Murdoch JN, The novel mouse mutant, chuzhoi, has disruption of Ptk7 protein and exhibits defects in neural tube, heart and lung development and abnormal planar cell polarity in the ear., 2010; BMC developmental biology. 10() 87. PMID: 20704721 | PMCID: PMC2930600
Professor of Auditory Neuroscience
Institute for Auditory Neuroscience & InnerEarLab
University Medical Center Göttingen
M.D. University of Jena
- 1997 Postdoc with E. Neher at the MPI for Biophysical Chemistry
Junior Group Leader at the at the MPI for Biophysical Chemistry, Göttingen
2001 Leader of the InnerEarLab and Clinical Work at the Department of
Otolaryngology, University Medical Center Göttingen
of the Institute for Auditory Neuroscience, University Medical Center Göttingen
and group leader at the MPIs for Experimental Medicine and Biophysical
Chemistry and the German Primate Center
Major Research Interests
Auditory Neuroscience - Synaptic Physiology and Pathophysiology - Audiology and
Our work focuses on the molecular anatomy, physiology and pathophysiology of
sound encoding and information processing in the auditory system as well as the
restoration of hearing by gene replacement therapy and optogenetic stimulation.
We combine various techniques to characterize synapses of hair cells and the
auditory brainstem from the molecular to the systems level. This way we have
contributed to the understanding of structure and function of auditory synapses
and initiated the concept of auditory synaptopathy. Towards restoration of
hearing we aim to establish virus-mediated gene replacement therapy of auditory
synaptopathy and pursue the optogenetic stimulation of auditory nerve for
improving the performance of the cochlear implant.
Selected Recent Publications
Wrobel C, Dieter A, Huet A, Keppeler D,
Duque-Afonso C, Vogl C, Hoch G, Jeschke M, Moser T (2018) Optogenetic
stimulation of cochlear neurons activates the auditory pathway and restores
auditory-driven behavior in deaf adult gerbils. Jul 11;10(449). pii: eaao0540
Neef J, Ohn TL, Urban NT, Frank T, Jean P,
Hell SW, Willig KI, Moser T (2018) Quantitative optical nanophysiology of Ca2+-signaling
at inner hair cell active zones. Nat commun, 18;9(1):290. doi:
Mager T, Lopez de la
Morena D, Senn V4,5, Schlotte J, D Errico A, Feldbauer K, Wrobel C, Jung S,
Bodensiek K, Rankovic V, Browne L, Huet A, Jüttner J1, Wood PG, Letzkus JJ,
Moser T, Bamberg E (2018) High frequency neural spiking and auditory signaling
by ultrafast red-shifted optogenetics. Nat Commun. 2018 May 1;9(1):1750. doi:
Anthony Wei Peng
Department SOM - Physiology
University of Colorado Denver - Anschutz Medical
1. F31DC009168 (PENG, ANTHONY WEI) ,Sep 1,
2007 - Aug 31, 2008, NIH/NIDCD, Inner Ear Hair Bundle Proteomics, Role:
2. F32DC010975 (PENG, ANTHONY WEI), May 1,
2010 - Apr 30, 2013,NIH/NIDCD, Second messenger regulation of hair cell
mechanotransduction, Role: Principal Investigator
3. K99DC013299 (PENG, ANTHONY WEI),Jul 1,
2013 - Jun 30, 2015, NIH/NIDCD, Molecular roles in active and passive mechanics
in cochlear hair bundles, Role: Principal Investigator
4. R00DC013299 (PENG, ANTHONY WEI),Aug 1,
2015 - Jul 31, 2018,NIH/NIDCD, Molecular roles in active and passive mechanics
in cochlear hair bundles, Role: Principal Investigator
5. R01DC016868 (PENG, ANTHONY WEI), Jun 7,
2018 - May 31, 2023, NIH/NIDCD, Molecular mechanisms of cochlear hair bundle
mechanics, Role: Principal Investigator
1. Effertz T, Becker L, Peng AW, Ricci AJ.
Phosphoinositol-4,5-Bisphosphate Regulates Auditory Hair-Cell
Mechanotransduction-Channel Pore Properties and Fast Adaptation. J Neurosci.
2017 11 29; 37(48):11632-11646. PMID: 29066559.
2. Kazmierczak M, Kazmierczak P, Peng AW,
Harris SL, Shah P, Puel JL, Lenoir M, Franco SJ, Schwander M. Pejvakin, a
Candidate Stereociliary Rootlet Protein, Regulates Hair Cell Function in a
Cell-Autonomous Manner. J Neurosci. 2017 03 29; 37(13):3447-3464. PMID:
3. Peng AW, Gnanasambandam R, Sachs F, Ricci
AJ. Adaptation Independent Modulation of Auditory Hair Cell Mechanotransduction
Channel Open Probability Implicates a Role for the Lipid Bilayer. J Neurosci.
2016 Mar 09; 36(10):2945-56. PMID: 26961949.
4. Peng AW, Ricci AJ. Glass Probe Stimulation
of Hair Cell Stereocilia. Methods Mol Biol. 2016; 1427:487-500. PMID: 27259944.
5. Nam JH, Peng AW, Ricci AJ. Underestimated
sensitivity of mammalian cochlear hair cells due to splay between stereociliary
columns. Biophys J. 2015 Jun 02; 108(11):2633-47. PMID: 26039165.
AW, Effertz T, Ricci AJ. Adaptation of mammalian auditory hair cell
mechanotransduction is independent of calcium entry. Neuron. 2013 Nov 20;
80(4):960-72. PMID: 24267652.
JC, Peng AW, Ricci AJ, Pruitt BL. Faster than the speed of hearing:
nanomechanical force probes enable the electromechanical observation of
cochlear hair cells. Nano Lett. 2012 Dec 12; 12(12):6107-11. PMID: 23181721.
M, Peng AW, Salles FT, Ricci AJ. Swept field laser confocal microscopy for
enhanced spatial and temporal resolution in live-cell imaging. Microsc
Microanal. 2012 Aug; 18(4):753-60. PMID: 22831554.
AW, Salles FT, Pan B, Ricci AJ. Integrating the biophysical and molecular
mechanisms of auditory hair cell mechanotransduction. Nat Commun. 2011 Nov 01;
2:523. PMID: 22045002.
K, Shin K, Diensthuber M, Peng AW, Ricci AJ, Heller S. Mechanosensitive hair cell-like
cells from embryonic and induced pluripotent stem cells. Cell. 2010 May 14;
141(4):704-16. PMID: 20478259.
AW, Ricci AJ. Somatic motility and hair bundle mechanics, are both necessary
for cochlear amplification? Hear Res. 2011 Mar; 273(1-2):109-22. PMID:
AW, Belyantseva IA, Hsu PD, Friedman TB, Heller S. Twinfilin 2 regulates actin
filament lengths in cochlear stereocilia. J Neurosci. 2009 Dec 02;
29(48):15083-8. PMID: 19955359.
13. Xu Z, Peng AW, Oshima K, Heller S. MAGI-1, a
candidate stereociliary scaffolding protein, associates with the tip-link
component cadherin 23. J Neurosci. 2008 Oct 29; 28(44):11269-76. PMID:
David W. Raible
Professor of Biological Structure
Adjunct Professor of Genome Sciences and of Biology
University of Washington
RESEARCH FOCUS: Development of sensory
structures in the zebrafish
The lateral line is a placode-derived
structure used by acquatic vertebrates to detect water flow. It consists of
groups of mechanosensory hair cells clustered into neuromasts on the surface of
We are interested in how neuromasts are
initially positioned in the head and body, and how new neuromasts are formed as
the animal grows.
SENSORY HAIR CELL DEATH AND REGENERATION
Zebrafish mechanosensory hair cells are
susceptible to environmental insult resulting in death, just as are those in
the mammalian inner ear.
We are using the genetics of the
zebrafish to identify modifiers of hair cell death. In addition, we
are studying how new hair cells regenerate after damage.
Fluorescent aminoglycosides reveal
intracellular trafficking routes in mechanosensory hair cells.
Hailey DW, Esterberg R, Linbo TH, Rubel
EW, Raible DW
The Journal of clinical investigation.
2017 Feb; 127 2: 472-486
Mitochondrial calcium uptake underlies
ROS generation during aminoglycoside-induced hair cell death.
Esterberg R, Linbo T, Pickett SB, Wu P,
Ou HC, Rubel EW, Raible DW
The Journal of clinical investigation.
2016 09; 126 9: 3556-66
Innervation regulates synaptic ribbons
in lateral line mechanosensory hair cells.
Suli A, Pujol R, Cunningham DE, Hailey
DW, Prendergast A, Rubel EW, Raible DW
Journal of cell science. 2016 06; 129
Robust regeneration of adult zebrafish
lateral line hair cells reflects continued precursor pool maintenance.
Cruz IA, Kappedal R, Mackenzie SM,
Hailey DW, Hoffman TL, Schilling TF, Raible DW
Developmental biology. 2015 Jun; 402 2:
There and back again: development and
regeneration of the zebrafish lateral line system.
Thomas ED, Cruz IA, Hailey DW, Raible DW
Wiley interdisciplinary reviews.
Developmental biology. ; 4 1: 1-16